Journal: Frontiers in Medicine

Article Title: Integrated Bioinformatic Analysis Reveals TXNRD1 as a Novel Biomarker and Potential Therapeutic Target in Idiopathic Pulmonary Arterial Hypertension

doi: 10.3389/fmed.2022.894584

Figure Lengend Snippet: Validation of TXNRD1 in clinical samples. (A) Gene expression of hub genes in GSE113439. (B) Serum TXNRD1 was decreased in IPAH patients. (C) The ability of serum TXNRD1 levels to diagnose IPAH was assessed using ROC curve analysis. (D,E) The relationship between serum TXNRD1 and mPAP as well as PVR analyzed by Spearman’s correlation analysis.

Article Snippet: TXNRD1 levels were determined using the human TrxR enzyme immunoassay kit (CSB-E09731h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Biomarker Discovery, Gene Expression

Journal: Frontiers in Medicine

Article Title: Integrated Bioinformatic Analysis Reveals TXNRD1 as a Novel Biomarker and Potential Therapeutic Target in Idiopathic Pulmonary Arterial Hypertension

doi: 10.3389/fmed.2022.894584

Figure Lengend Snippet: In vitro and in vivo experimental validation and GSEA analysis of TXNRD1. (A) The hemodynamic data and HE staining of lung tissue in animal experiments. (B) Representative Western blots and quantification of TXNRD1 and β-actin in the lungs of monocrotaline treated rats and controls ( n = 7 each, * P < 0.05), data represent the mean ± SEM and Student t -test was used to compare two groups. (C) Immunofluorescence images of lung distal PA from MCT rats and controls. (D) Representative Western blots and quantification of TXNRD1 levels in rat PASMCs under PDGF-BB stimulation (30 ng/ml) normalized to a β-actin internal control ( n = 3 each,* P < 0.05). (E) Venn diagram of GSEA terms among five datasets based on correlation analysis of TXNRD1. (F) The overlapping three GSEA terms correlated with TXNRD1. **** P < 0.0001.

Article Snippet: TXNRD1 levels were determined using the human TrxR enzyme immunoassay kit (CSB-E09731h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: In Vitro, In Vivo, Biomarker Discovery, Staining, Western Blot, Immunofluorescence, Control

Journal: Frontiers in Medicine

Article Title: Integrated Bioinformatic Analysis Reveals TXNRD1 as a Novel Biomarker and Potential Therapeutic Target in Idiopathic Pulmonary Arterial Hypertension

doi: 10.3389/fmed.2022.894584

Figure Lengend Snippet: Silencing of TXNRD1 exacerbated PDGF-induced PASMC malignant phenotype. (A) Knockdown efficiency of TXNRD1 was verified by qPCR and Western Blot. (B) PASMCs were transfected with siRNA for 48 h before treatment with PDGF-BB (20 ng/ml) for another 24 h, and western blotting was used to detect proliferative and apoptotic markers in cell lysates. (C) Normalized quantification of cleaved-PARP, BAX/Bcl-2 and PCNA expression ( n = 3 each, * P < 0.05). (D) Cell proliferative ability was determined by EdU assay in siTXNRD1-PASMC under PDGF stimulation. (E) Calculation of EdU-stained cells rate ( n = 6 each, * P < 0.05). (F) Cell migration was measured by scratch assay in siTXNRD1-PASMC under PDGF stimulation. (G) Calculation of scratch closed percentage ( n = 6 each, * P < 0.05). Data represent the mean ± SEM. Student t -test and one-way ANOVA were used to compare two and multiple groups. ** P < 0.01, *** P < 0.001,**** P < 0.0001.

Article Snippet: TXNRD1 levels were determined using the human TrxR enzyme immunoassay kit (CSB-E09731h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Knockdown, Western Blot, Transfection, Expressing, EdU Assay, Staining, Migration, Wound Healing Assay

Journal: BMC Pharmacology & Toxicology

Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

doi: 10.1186/s40360-026-01225-w

Figure Lengend Snippet: Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system (Trx1/TrxR1) ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant

Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

Techniques: Expressing

Journal: BMC Pharmacology & Toxicology

Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

doi: 10.1186/s40360-026-01225-w

Figure Lengend Snippet: Epinephrine and EGCG effect on cell viability, Nrf2, NTF, and TrxR1 expression in hepatoma cells. HepG2 and Huh7 cells were treated with epinephrine ( A ) or EGCG ( B ) for 24 h, after which cell metabolic activity was determined by MTT assay. Epinephrine enhanced cell viability in both HepG2 and Huh7 cells, whereas EGCG reduced cell viability. ( C ), ( D ), and ( E ) show the protein levels of Nrf2, TNF-α, and TrxR1, respectively, assessed in cells treated with EP, EGCG, CPZ, EP/CPZ, or EGCG/CPZ. Bars represent the mean (± SD) of 3 experiments. Groups were compared using ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, (***): p < 0.001, (****): p < 0.0001, and “ns” is not significant

Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

Techniques: Expressing, Activity Assay, MTT Assay

Journal: BMC Pharmacology & Toxicology

Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

doi: 10.1186/s40360-026-01225-w

Figure Lengend Snippet:

Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

Techniques:

Journal: Frontiers in Immunology

Article Title: Tumor cells express and maintain HMGB1 in the reduced isoform to enhance CXCR4-mediated migration

doi: 10.3389/fimmu.2024.1358800

Figure Lengend Snippet: Endogenous redox status of HMGB1 in cancer cells, and release of HMGB1, Trx and TrxR1 in the extracellular milieu. (A) Annotated MS/MS spectra of the most abundant peptide forms containing either the light (blue) or heavy (red) iodoacetamide derivative-modified form of each of the HMGB1 cysteines identified with the recombinant full length HMGB1. Green and orange lines represent the annotated fragment peaks for each MS/MS spectrum, where green marks the b-ion series and orange marks the y-ion series. The resulting fragmentation pattern is reported on the peptide sequence with the same color code to visualize the peptide spectrum match. In grey the common methionine oxidation modification is depicted. Andromeda identifications are visualized with PDV ( http://pdv.zhang-lab.org ). (B) Percentage of reduced and oxidized HMGB1 based on the redox status of C23 and C106 in MCF-7 (n=3), MDA-MB-231 (n=3), PC-3 (n=3), and in CD3 + T cells from healthy donors (n=2). Data are shown as mean + SEM of the independent experiments performed. (C) HMGB1 concentration detected by ELISA in the cell culture supernatants. Data present the mean ± SEM of 3 independent experiments. (D) qRT-PCR analysis (normalized on 18S ) is shown for TRX and TRXR1 mRNA in cancer cells. Data are shown as mean ± SEM of 3 independent experiments. (E) Concentration of Trx and TrxR1 detected by ELISA in the cell culture supernatants. Data present the mean ± SEM of 3 independent experiments. Statistical analysis of the differences among the cell lines was performed using one way ANOVA, followed by Tukey’s multiple comparison test: *p < 0.05.

Article Snippet: The detection of HMGB1, thioredoxin, thioredoxin reductase 1, or CXCL12 in cancer cell supernatants was performed by ELISA following the manufacturer’s instructions: HMGB1 Express ELISA kit (30164033, TECAN), Trx (EK1254, Boster Biological Technology Co), TrxR1 (NBP2-68164, Novus Biologicals), and CXCL12 (DY350, R&D Systems).

Techniques: Tandem Mass Spectroscopy, Modification, Recombinant, Sequencing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Quantitative RT-PCR, Comparison